[SA_Scat] ACA 2018 - Small Angle Scattering Workshop

[Kushol Gupta]

"Applications of Small Angle Scattering to Structural Biology: An
Introduction." 

 

On behalf of the organizing committee, we are pleased to announce a workshop
on "Applications of Small Angle Scattering to Structural Biology: An
Introduction." 

 

This workshop will take place on Friday, July 20th, 2018 at this year's
American Crystallographic Association (ACA) Meeting in Toronto, Canada. The
meeting itself will run from July 20-24.

Over the past two decades, SAS has become a mainstay technique for the study
of structure and composition in solution for structural biologists around
the world. In contrast to the more intensive SAS courses in Europe that
extend across several days, this single-day workshop will be comprised of
carefully constructed lectures and tutorials to serve as an introduction to
investigators new to the technique. The workshop format will include
lectures and a selection of hands-on practical exercises.  Throughout the
workshop the emphasis will be on practical application: knowing how to judge
data quality, how to troubleshoot during data collection, and the
expectations for a successful experiment and acceptable publication.
Students will also learn about aspects of home laboratory data collection
and will be introduced to experiments at national user facilities
(synchrotrons and research reactors).   

 

Students will be expected to bring laptops with the appropriate
pre-installed software. Extra laptops will be provided in case of unexpected
hardware or software issues on-site, and network connectivity will be
provided as part of the course, as some exercises and tutorials will reply
on external computer-server resources. Preloaded portable disks and memory
sticks will be provided to help reduce the need for large downloads over
conference bandwidth.

We thank Anton-Paar, Rigaku Corporation, SAXSLAB, and Wyatt Technology
Corporation for their kind support of this event.

Tentative Program Schedule

Time      Topic

8:00 AM    Registration and Software Installation Help; Speaker Introduction

8:30 AM    BioSAS: Overview 

Why is SAS helpful?

9:00 AM    The Scattering Profile 

Guinier analysis, concentration, monodispersity, etc.

9:30 AM    Tutorial 1: Basic Data Analysis I with RAW 

Is this good data? Radiation Damage?

10:00 AM   Coffee Break

10:15 AM   Data Analysis: Model-independent Features 

Molecular Weight Determination, Invariant Plots and Flexibility, The P(r)
function

11:00 AM   Tutorial 2: Basic Data Analysis II with RAW 

Calculate a P(r) function, determine Molecular Weight, Dmax, Rg, Volume,
etc.

12:00 PM   Working Lunch - Laboratory Source SAXS 

Discussion with Scientists, Experimental Advice

1:00 PM    SEC-SAXS 

Application and Practical Considerations

1:30 PM    Sample Preparation

Sample purification and preparation, aggregation, buffer selection, etc

1:45 PM    Complementary Biophysical Methods for Sample Assessment 

The importance of supporting biophysical methods including static and
dynamic light scattering and analytical ultracentrifugation  

2:00 PM    Data Analysis: Model-dependent Analysis of SAS Data

Calculated scattering profiles from atomic models, Shape reconstruction,
Atomistic modeling

2:30 PM    Tutorial 3: Three-dimensional modeling from SAS data

3:15 PM    Coffee Break

3:30 PM    Guest Research Lecture - Thomas Grant, Research Asst. Professor,
University of Buffalo/Hauptman-Woodward Medical Research Institute

4:30 PM    SANS and Contrast Variation 

5:00 PM    Publishing SAS data 

5:30 PM    Conclusion

Pre-registration is mandatory. The fee to attend is: 

.             Student (undergrad, grad or postdoctoral) - $185 (CAN)

.             Corporate -  $355 (CAN)

.             Non-corporate or non-student - $185 (CAN)

Lunch will be included.

Contact: 

Kushol Gupta, Perelman School of Medicine, University of Pennsylvania.
kgupta at upenn.edu

Confirmed Instructors:

Angela Criswell, Rigaku Corporation

Srinivas Chakravarthy, BioCAT Group, Advanced Photon Source

Richard Gillilan, Macromolecular Diffraction Facility, Cornell High Energy
Synchrotron Source

Thomas Grant, University of Buffalo/Hauptman-Woodward Medical Research
Institute

Jesse Hopkins, BioCAT Group, Advanced Photon Source

Maxim Petoukhov, BIOSAXS Group, EMBL Hamburg

More information is available at:

http://www.amercrystalassn.org/2018-workshops   

http://www.amercrystalassn.org/2018-meeting-homepage 

 

 

 

Kushol Gupta, Ph.D.

Research Assistant Professor,
<https://www.med.upenn.edu/biocbiop/index.html> Department of Biochemistry
and Biophysics

Director,  <https://www.med.upenn.edu/biocbiop/jf/bsbcore/index.html>
Johnson Foundation Structural Biology and Biophysics Core

 <http://www.med.upenn.edu/> Perelman School of Medicine at
<http://www.upenn.edu/> The University of Pennsylvania

 <mailto:kgupta at upenn.edu> kgupta at upenn.edu / 267.259.0082 /
<http://www.stwing.upenn.edu/~kgupta> www.stwing.upenn.edu/~kgupta