[SA_scat] protein SAXS in conc Zn soln
Richard Gillilan reg8 at cornell.eduThu Sep 7 15:11:09 BST 2006
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I'm cross posting this on CCP4 and SA_scat, so sorry if you get two copies. One of my users wants to do SAXS on a protein in a buffer containing concentrated Zn ions. I did some simple calculations of contrast and the results make me worry. water = 334 electrons/nm^3 protein = 430 electrons/nm^3 For saturated ZnCl2, I estimate 530 electrons/nm^3 He didn't specify what kind of Zn salt, but it seems that any addition of Zn to the buffer is going to initially decrease contrast. At best, in a very strong solution, there would be negative contrast about equal to water in magnitude. Has anyone had experience of doing SAXS in concentrated heavy atom solutions? Did you have contrast problems? Richard Gillilan MacCHESS Cornell
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